Linda M. Kohn

kohn.jpg
Full Professor
Education: 
Ph.D., Cornell University, 1979
Office phone: 
905-828-3997
Lab phone: 
905-828-5338
Fax: 
905-828-3792
Office: 
DV 3047
Lab: 
DV 4090C

linda [dot] kohn (at)utoronto [dot] ca

Address: 

Department of Biology
South Building, Room 3047
University of Toronto
3359 Mississauga Road North
Mississauga, Ontario
Canada L5L 1C6

Research: 

My research has been in three areas. 1. Taxonomy and evolutionary systematics. My specialty is ascomycetes, especially the discomycetes (cup fungi). Most specifically, I continue to study the Sclerotiniaceae, a family showing a fascinating array of trophic relationships with plants, some of which are insect-mediated. I use both molecular and morpho-taxonomic approaches. 2. Population processes of fungi associated with agricultural (crops and weeds) or wild plants. I am interested in both population structure and the underlying genetic and phenotypic features of populations. For example, we have identified the emergence of high temperature competence in clonal lineages of a plant parasite after successive years of elevated temperatures and drought. Work in my lab can be either retrospective or experimental. The retrospective studies are based on our sampling from the field from which we infer historic or contemporary relationships that test a hypothesis. Work with the samples starts with multi-locus gene genealogies based on DNA sequences, adds fingerprint and microsatellite data, and explores statistically a wide variety of associations of genotypes,e.g., with geography at scales ranging from global to local, with host or host symptoms, and with genome size. We do a range of experimental studies, from testing phenotype - genotype associations to experimental evolution in which we evolve populations under selection and observe the emergence and increase in frequency of adaptive mutations in real time. We are currently using both experimental evolution and retrospective studies based on field sampling to determine to what extent mechanisms conveying fungicide resistance in experimental populations of yeast pertain to field populations of Botrytis cinerea and Sclerotinia sclerotiorum. 3. I am collaborating with James B. Anderson and Postdoctoral Fellow, Jeremy Dettman on experimental evolution of speciation, which, like our work on drug resistance, will elucidate both process and mechanisms.

2004
Carbone, I, Kohn, L.M. (2004).  Inferring process from pattern in fungal population genetics.. Fungal Genomics, Applied Mycology and Biotechnology Series.. Abstract
2003
Anderson, J., Sirjusingh, C., Parsons, A., Boone, C., Wickens, C., Cowen, L., Kohn, L. (2003).  Mode of Selection and Experimental Evolution of Antifungal Drug Resistance in Saccharomyces cerevisiae. Genetics. 163, 1287-1298. Abstract
2002
Phillips, D.V., Carbone, I, Gold, S.E., Kohn, L.M. (2002).  Phylogeography and genotype - symptom associations in early and late season infections of canola by Sclerotinia sclerotiorum.. Phytopathology. 92 (7), 785-793. Abstract
Cowen, L.E., Anderson, J.B., Kohn, L.M. (2002).  Evolution of drug resistance in Candida albicans.. Annual Review of Microbiology. 56, 139?165. Abstract
Couch, B.C., Kohn, L.M. (2002).  A multilocus gene genealogy concordant with host preference indicates segregation of a new species, Magnaporthe oryzae, from M. grisea.. Mycologia. 94(4), 683-693. Abstract
Hambleton, S., Walker, C., Kohn, L.M. (2002).  Clonal lineages of Sclerotinia sclerotiorum previously known from other crops predominate in 1999-2000 samples from Ontario and Quebec soybean.. Canadian Journal of Plant Pathology. 24, 309-315. Abstract
2001
Sirjusingh, C., Kohn, L.M. (2001).  Characterization of microsatellites in the fungal plant pathogen, Sclerotinia sclerotiorum.. Molecular Ecology Notes. 1, 267-269.
Carbone, I, Kohn, L.M. (2001).  A microbial population-species interface: nested cladistic and coalescent inference with multilocus data.. Molecular Ecology. 10, 947-967. Abstract
Carbone, I, Kohn, L.M. (2001).  Multilocus nested haplotype networks extended with DNA fingerprints show common origin and fine-scale, ongoing genetic divergence in a wild microbial metapopulation.. Molecular Ecology. 10, 2409-2422. Abstract
Gernandt, D.S., Platt, J.L., Stone, J.K., Spatafora, J.W., Holst-Jensen, A., Hamelin, R.C., Kohn, L.M. (2001).  A nuclear ribosomal DNA small subunit phylogeny of the Helotiales and Rhytismatales.. Mycologia. 93, 915-933.
2000
Couch, B.C., Kohn, L.M. (2000).  Clonal spread of Sclerotium cepivorum in onion production with evidence of past recombination events.. Phytopathology. 90, 514-521.
Cowen, L.E., Sanglard, D., Calabrese, D., Anderson, J.B., Kohn, L.M. (2000).  Evolution of drug resistance in experimental populations of Candida albicans.. Journal of Bacteriology. 182, 1515-1522. Abstract
1999
Carbone, I, Anderson, J.B., Kohn, L.M. (1999).  Patterns of descent in clonal lineages and their multilocus fingerprints are resolved with combined gene genealogies.. Evolution. 53, 11 . Abstract
1998
Anderson, J.B., Kohn, L.M. (1998).  Genotyping, gene genealogies and genomics bring fungal population genetics above ground.. Trends in Ecology and Evolution. 13, 444-449. Abstract
Kohli, Y., Kohn, L.M. (1998).  Detection of random association among loci in clonal populations of Sclerotinia sclerotiorum.. Fungal Genetics and Biology. 23, 139-149.
1997
Cubeta, M.A., Cody, B.C., Kohli, Y., Kohn, L.M. (1997).  Clonality in Sclerotinia sclerotiorum on infected cabbage in eastern North Carolina.. Phytopathology. 87, 1000-1004.
Holst-Jensen, A., Kohn, L.M., Schumacher, T. (1997).  Nuclear rDNA phylogeny of the Sclerotiniaceae.. Mycologia. 89, 885-899.
Holst-Jensen, A., Kohn, L.M., Jakobsen, K.S., Schumacher, T. (1997).  Molecular phylogeny and evolution of Monilinia (Sclerotiniaceae) based on coding and non-coding rDNA sequences.. American Journal of Botany. 84, 686-701.
1996
Errampalli, D., Kohn, L.M. (1996).  Electrophoretic karyotypes of Sclerotinia sclerotiorum.. Applied and Environmental Microbiolology. 62, 4247-4251.
Kohli, Y., Kohn, L.M. (1996).  Mitochondrial haplotypes in populations of the plant-infecting fungus Sclerotinia sclerotiorum: wide distribution in agriculture, local distribution in the wild.. Molecular Ecology.. 5, 773-783.
1995
Kohli, Y., Brunner, L.J., Yoell, H., Milgroom, M.J., Anderson, J.B., Morrall, R.A.A., Kohn, L.M. (1995).  Clonal dispersal and spatial mixing in populations of the plant pathogenic fungus, Sclerotinia sclerotiorum.. Molecular Ecology. 4, 69-77.
Carbone, I, Anderson, J.B., Kohn, L.M. (1995).  A group I intron in the mitochondrial small subunit rRNA gene of Sclerotinia sclerotiorum.. Current Genetics. 27, 166-176.
Anderson, J.B., Kohn, L.M. (1995).  Clonality in soilborne, plant-pathogenic fungi.. Annual Review of Phytopathology. 33, 369-391.
Kohn, L.M. (1995).  The clonal dynamic in wild and agricultural plant pathogen populations.. Canadian Journal of Botany. 73 (Suppl., S1231-S1240.